저출력 레이저조사가 배양섬유아세포의 생존력에 끼치는 영향에 관한 실험적 연구
AN EXPERIMENTAL STUDY O THE EFFECTS OF LOW LEVEL LASER IRRADIATION OFN THE CELL VIABILITY OF CULTURED FIBROBLAST
양근영, 한경수, 강세숙,
소속 상세정보
양근영 ( ) - 원광대학교
한경수 ( ) - 원광대학교
강세숙 ( ) - 원광대학교
KMID : 0355219930180020097
Abstract
This study was performed to investigate the effects of infrared and visible light laser irradiation on cell viability of human gingival fibroblast. For the present study, the author used cultured fibroblast originated from sound gingiva which
were
fifth
or sixth passage. Laser machine utilized here were stomalaser which irradiate infrared(GaAs diode) and red(HeNe) laser in turn with pulse wave pattern or continuous wave pattern, and the machine had several frequency mode presented by
regeneration,
relaxation and analgesic modalities.
Cultured fibroblast samples were divided by this modalities or cell counts and laser exposure time which were 60 seconds or 150 seconds, respectively.
1 day after laser irradiation, each cell-well was treated with MTT and measured optical density with ELISA.
@ES The obtained results were as follows :
@EN 1. There was a tendency of increasing optical density in proportion to irradiation time in groups of 1×10E4 cell per well but in group 5×10E3 cell per well, reverse phenomena were observed.
2. The difference of optical density according to frequency modalities were not showed significantly except several cases in groups of 5×10E3 cell per well.
3. In general, cell viability of cultured human gingival fibroblast were not showed consistent feature by low level laser irradiation.
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